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Key specific Scalp is: acupuncture Bidirectional adjustment

Acute cerebral hemorrhage of the fail at anything

Nerve electro-physiology

 

 

Acupuncture two-way regulating functions is when the human body function in the state, acupuncture unbalanced developments to the body, some are the imbalance of the function can be transformed to the normal state of equilibrium [1]. Neurons electrical activity is directly reflect the central nerve system function of the state is an important index. Acute cerebral hemorrhage, neurons the electrical activity of the affected, the function is restrained. Head point acupuncture treatment of acute cerebral hemorrhage curative effect is distinct, plenty of clinical observation and experiment have been proved head point acupuncture has obvious neurons protection [2-6]. This experiment used micro electric great nerve cells in the brain hemorrhage discharge technology, head back and forth like acupuncture point the incubation period of reflection and discharge the bidirectional effect change neurons, from the nerve cells to explore electrophysiological head of acupuncture point two-way adjusting function and mechanism.

1 materials and methods

1.1 animals and group

Male Wistar rats (225 ~ 250 g) only by 40, heilongjiang university of traditional Chinese medicine laboratory animal center to provide. Part of the random digital PEN method is divided into four groups: normal group (n = 8), salt water group (n = 8), note blood group (n = 8), acupuncture group (n = 8). According to random number and PIN part method is divided into four groups: normal group (n = 2), salt water group (n = 2), note blood group (n = 2), acupuncture group (n = 2).

1.2 model preparation

The intraperitoneal injection ec was 20% (1 g/kg) anesthesia. The model used their own nuclear bleeding left ventricular artery whole blood into the law, note HP 30 edged. This method for our since 1998 for cerebral hemorrhage animal studies to build mode method, simple operation, high success rate, made the sign is the behavior model success with the hand: the animal tail mentioned the hind legs from the ground, the animal to the fore as the center where palsy delimit circle. Nuclei positioning according to the Pellegrino map and the tail nuclear A (1.0 ~ 1.8); L (2.6 ~ 3.4); H (5.0 ~ 7.0) by the beam nuclear A: (2.2 ~ 2.6); L (1.0 ~ 1.4); H (5.5 ~ 6.5). In after the curative immediately blood note will be through the temple area. MaYi electricity for G-6805 type, pick up the positive, negative by the sun. Choose parameters for 1 v, 15 Hz ac power for 10 minutes, and time. Salt water into the same amount of 0.9% of the physiological saline, the rest step with note blood group.

1.3 test methods

According to the tail of the radiant heat discharge method, gathered in diameter 1 cm, control guy in 4 ~ reflection incubation period between 8.30 seconds. Choose response to pain neurons to observe object, as the radiant heat start and guy reflex occurs when electricity is stop illuminate stimulator use single pulse marked with pain, mark excited neurons (PEN) and pain suppression neurons (PIN) discharge situation shows at the same time in the VC-9 oscilloscope, at the same time use the GF 777 double ways for the tape recorder, and finally the record-lab chart4.0 Power medical data processing software to convert discharge frequency chart, through the frequency of numerical calculation on the drawing a latent period, inhibit schedule and after JingZengZhi analysis.

1. 4 statistics processing

The experimental results by SPSS10.0 statistical software t test in statistical analysis.

2 the results

2.1 the acupuncture to rat different state guy (TFL) influence of incubation period

2.1.1 of normal rats acupuncture TFL, the influence of see table 1.

Table 1 of normal rats acupuncture TFL (unit: S) influence (X + SD) comparison

Time window (the minutes) normal group (8) the acupuncture group (only 8 only)

Acupuncture 10-0 before 5.16 + 0.15 + /-0.13 5.15

Cupping 10 minutes

After acupuncture 0-3 5.15 + 0.12 + /-0.15 7.65 ▲ ▲

After acupuncture 4-6 5.18 + 0.17 + /-0.16 7.32 ▲ ▲

After acupuncture 7-9 5.20 + 0.14 + /-0.20 6.85 ▲ ▲

Acupuncture group group compared with normal ▲ P < 0.05 ▲ ▲ P < 0.01

2.1.2 acupuncture to cerebral hemorrhage rat TFL, the influence of see table 2.

Table 2 acupuncture to cerebral hemorrhage rat TFL (unit: S) influence (plus or minus the comparison of the SD)

Time window (the minutes) normal group (8) the acupuncture group (only 8 only)

Note 10-0 before blood 5.60 + 0.13 + /-0.18 5.61

Note the blood 0-after 10 + /-0.19 5.75 in the local area for 10 minutes

Note 11-20 after blood 6.05 + 5.69 + /-0.12 0.17 * * ▲ ▲

Note the blood after 21-30 + 6.34 0.17 5.86 + 0.13 * * * ▲ ▲

Each group and note before the blood is * P < 0.05 * * P < 0.01

Acupuncture group and the blood group compared ▲ P < 0.05 ▲ ▲ P < 0.01

From the table 1 and 2, visible, head of acupuncture point normal rats TFL has inhibition (P < 0.01). And when note blood guy rats reflection is restrained, TFL obvious when extended (P < 0.01), head of acupuncture point acupuncture can make by inhibiting the guy, and restore reflection of the extended TFL shortened (P < 0.01), namely the acupuncture can reduce blood to rat TFL to note, and the influence of the immediate effects significant.

2.2 the acupuncture to acute cerebral hemorrhage rat left end (local) nuclear hematoma response to pain neurons the influence of discharge (see table 3-4)

Table 3 each rat different time window left the hospital discharge more nuclear PEN SD)

Time window index number of normal saline groups note blood group acupuncture group

Note the blood (water) incubation period (S) before 8 4.73 + 0.16 4.71 4.79 + 0.14 + 0.19 + /-0.20 4.75

30-21 minutes JingZengZhi

(9.90 + /-1.16 Hz ac) 9.70 + 1.30 + 1.29 + /-1.27 9.24 9.87

Note the blood (water) incubation period (S) before 8 4.80 + 0.19 4.92 4.83 + 0.23 + /-0.22 in the local area for 10 minutes

20-11 minutes JingZengZhi (9.40 + /-1.34 Hz ac) 8.78 + 1.37 + /-0.90 9.17

Note the blood (water) incubation period (S) before 8 4.79 + 0.14 5.21 4.65 + 0.23 + /-0.20 5.67 + /-0.16 * *

10-0 minutes JingZengZhi (9.24 + /-1.29 Hz ac) 8.40 + 1.97 + 0.92 + /-1.35 8.91 6.95 * *

Note the blood (water) after the incubation period (S) and 4.85 + 0.16 5.12 5.16 + 0.23 + /-0.22 * 10 minutes in the local area

0-10 minutes JingZengZhi (9.10 + /-1.46 Hz ac) 8.51 + 1.94 5.21 + /-0.90 * *

Note the blood (water) after the incubation period (S) and 4.87 + 0.19 5.07 6.21 + 0.14 + 5.27 + /-0.36 0.19 * * * ▲ ▲

11-20 minutes JingZengZhi (8.46 + /-0.97 Hz ac) 8.92 + 1.94 1.77 0.55 + 6.95 + 1.35 * * * * * * * ▲ ▲

Note the blood (water) after the incubation period (S) and 4.87 + 0.19 5.07 7.03 + 0.14 + 6.31 + /-0.24 0.17 * * * * * * * ▲ ▲

21-30 minutes JingZengZhi (8.46 + /-0.97 Hz ac) 8.92 + 1.94 0.30 0.21 + 2.15 + 1.02 * * * * * * * ▲ ▲

Each group and put before treatment factor comparison * P < 0.05 * * P < 0.01

Acupuncture group and the blood group compared ▲ P < 0.05 ▲ ▲ P < 0.01

Table 4 each rat different time window left end nuclear PIN discharge more (+ SD)

Time window index number of normal saline groups note blood group acupuncture group

Note the blood suppress schedule before (S) 2 2.3 + 0.36 2.37 2.32 + 0.24 + 0.31 + /-0.31 2.35

30-21 minutes JingZengZhi (Hz ac)-7.65 + /-1.12-7.53 + /-0.89-7.47 + /-0.78-7.50 + 0.72

Note the blood suppress schedule before (S) 2 2.28 + 0.29 2.32 2.34 + 0.22 + /-0.22 in the local area for 10 minutes

20-11 minutes JingZengZhi (Hz ac)-7.40 + /-1.34-7.58 + /-1.07-7.35 + 1.09

Note the blood suppress schedule before (S) 2 2.39 + 0.24 2.29 2.35 + 0.21 + /-0.20 1.27 + /-0.16 * *

10-0 minutes JingZengZhi (Hz ac)-7.24 + /-1.29-7.40 + /-1.17-7.39 + /-0.52-5.95 + 1.55 * *

Note the blood after schedule suppression (S) 2 2.27 + 0.45 2.05 3.16 + 0.26 + /-0.43 * 10 minutes in the local area

0-10 minutes JingZengZhi (Hz ac)-7.36 + /-0.74-6.65 + /-0.55-8.78 + 0.67 * *

Note the blood after schedule suppression (S) 2 2.23 + 0.42 2.01 3.66 + 0.28 + 3.16 + /-0.17 0.56 * * * * * * * ▲ ▲

11-20 minutes JingZengZhi (Hz ac)-7.29 + /-1.02-6.39 + 1.03 9.8 0.41 + 8.89 + 0.62 * *-* * ▲ ▲

Note the blood after schedule suppression (S) 2 2.21 + 0.33 1.95 4.17 + 0.19 + 2.94 + /-0.12 0.07 * * * * * * * ▲ ▲

21-30 minutes JingZengZhi (Hz ac)-7.26 + /-0.93-6.25 + 0.86 11.7 0.16 + 8.03 + 0.52 * *-* * ▲ ▲

Each group and put before treatment factor comparison * P < 0.05 * * P < 0.01

Acupuncture group and the blood group compared ▲ P < 0.05 ▲ ▲ P < 0.01

2.3 the acupuncture to acute cerebral hemorrhage rat left by the beam nuclear (site) response to pain hematoma neighboring neurons the influence of discharge (see table 5-6)

Table 5 each rat different time window of nuclear PEN discharge the left is + SD)

Time window index number of normal saline groups note blood group acupuncture group

Note the blood incubation period (S) before 8 4.13 + 0.19 4.11 4.12 + 0.17 + 0.19 + /-0.17 4.13

30-21 minutes JingZengZhi (9.60 + /-1.14 Hz ac) 9.70 + 1.28 + 1.18 + /-1.37 9.65 9.75

Note the blood incubation period (S) before 8 4.20 + 0.17 4.20 4.09 + 0.12 + /-0.13 minutes in the local area

20-11 minutes JingZengZhi (9.40 + /-1.24 Hz ac) 9.68 + 1.31 + /-1.19 9.67

Note the blood incubation period (S) before 8 4.27 + 0.14 4.21 4.25 + 0.18 + /-0.20 6.07 + /-0.36 * *

10-0 minutes JingZengZhi (9.54 + /-1.09 Hz ac) 9.45 + 1.27 + 1.02 + /-1.15 9.69 6.95 * *

Note the blood after incubation period (S) and 4.23 + 0.11 4.19 4.31 + 0.14 + /-0.16 minutes in the local area

0-10 minutes JingZengZhi (9.37 + /-1.24 Hz ac) 9.00 + 1.21 + /-1.12 7.05 *

Note the blood after incubation period (S) and 4.29 + 0.23 4.30 4.89 + 0.15 + 4.34 + /-0.11 0.18 * * * ▲ ▲

11-20 minutes JingZengZhi (9.49 + /-1.22 Hz ac) 8.75 + 1.16 6.70 1.15 + 8.65 + 1.36 * * * * * * * ▲ ▲

Note the blood after incubation period (S) and 4.31 + 0.22 4.51 6.07 + 0.19 + 5.12 + /-0.17 0.20 * * * * * * * ▲ ▲

21-30 minutes JingZengZhi (9.56 + /-1.33 Hz ac) 8.63 + 1.24 5.14 1.16 + 7.95 + 1.29 * * * * * * * ▲ ▲

Each group and put before treatment factor comparison * P < 0.05 * * P < 0.01

Acupuncture group and the blood group compared ▲ P < 0.05 ▲ ▲ P < 0.01

Table 6 each rat different time window of nuclear PIN discharge the left (+ SD) comparison

Time window index number of normal saline groups note blood group acupuncture group

Note the blood suppress schedule before

(S) 2 2.25 + 0.09 2.27 2.22 + 0.07 + 0.10 + /-0.08 2.23

30-21 minutes JingZengZhi (Hz ac)-8.25 + /-0.82-8.2 + /-1.15-8.27 + /-0.28-8.29 + 1.44

Note the blood suppress schedule before

(S) 2 2.27 + 0.06 2.21 2.24 + 0.10 + /-0.07 in the local area for 10 minutes

20-JingZengZhi 11 minutes

(Hz ac)-8.11 + /-1.03-8.19 + /-1.23-8.13 + 1.09

Note the blood suppress schedule before

(S) 2 2.28 + 0.08 2.22 2.25 + 0.13 + /-0.12 1.27 + /-0.16 * *

10-0 minutes JingZengZhi

(Hz ac)-7.86 + /-0.74-7.94 + /-1.17-8.09 + /-0.52-5.95 + 1.55 * *

Note after blood suppress schedule

(S) 2 2.29 + 0.10 2.15 2.86 + 0.06 + /-0.07 * 10 minutes in the local area

0 JingZengZhi-10 minutes

(Hz ac)-8.29 + /-0.92-7.85 + /-0.75-9.50 + 0.67 * *

Note after blood suppress schedule

(S) 2 2.26 + 0.73 2.13 3.27 + 0.09 + 2.71 + /-0.07 0.05 * * ▲ ▲

11-20 minutes JingZengZhi

(Hz ac)-8.16 + /-1.12-7.13 + 1.08 10.2 0.67 + 8.94 + 0.52 * *-▲ ▲

Note after blood suppress schedule

(S) 2 2.23 + 0.06 2.06 3.76 + 0.06 + 2.58 + /-0.05 0.15 * * * * * * * ▲ ▲

21-30 minutes JingZengZhi

(Hz ac)-8.23 + /-0.12-7.35 + 0.69 11.1 0.67 + 8.52 + 0.64 * *-* * ▲ ▲

Each group and put before treatment factor comparison * P < 0.05 * * P < 0.01

Acupuncture group and the blood group compared ▲ P < 0.05 ▲ ▲ P < 0.01

From the table 3, 4 and table 5, and 6 visible, left and left by the end of the nuclear nuclear brine group in the note saline after the time window PEN and PIN discharge and note before the brine non-significance difference (P > 0.05), indicating that the operation of the process the impact is not big. The left end of the nuclear and left nuclear note blood group in the note blood after the time window discharge and the blood PEN than before, JingZengZhi reduce extended incubation period (P < 0.05, P < 0.01), PIN discharge and the blood than before when extended, JingZengZhi inhibit process to reduce (P < 0.01), indicating that note blood PEN and PIN to radiate heat after according to the tail of the pain from stimuli drop, namely the excitability of PEN and PIN inhibiting decreased, and have with the extension of time and aggravation of the trend, different is neurons discharge inhibition degree left end of heavy, nuclear left by a nuclear light. The left end of the nuclear and left nuclear acupuncture group in note blood PEN after acupuncture incubation period before extended, JingZengZhi reduce (P < 0.01); PIN in the note blood after acupuncture before schedule shorten, JingZengZhi inhibit increased (P < 0.01), this shows that acupuncture can make the physical condition of pain in the excitement of the PEN stimulate gender to drop, PIN to pain stimulus, to play the inhibitory improve analgesic action. And the acupuncture group in the note after blood after acupuncture group compared with note blood were extremely significant difference (P < 0.01), to extend the incubation period and curb schedule, reduce JingZengZhi increase the shortened that acupuncture can make blood by inhibiting the influence and the PEN of irritability and PIN inhibiting are restored, namely the acupuncture can reduce blood on the left end nuclear injection and left the PEN and PIN of nuclear discharge, and inhibition immediate effects significant. Different is head of the acupuncture point left beam nuclear neurons discharge recovery degree is left end nuclear neurons obvious.

3 discuss

According to the tail as radiant heat damage sexual stimulation induce guy rats reflection is measured the classical theory of pain, is now experiments show that discharge incubation period and guy incubation period between the stable relationship [8 to 9]. PEN and PIN and TFR directly related changes have taken place in the discharge, neurons TFR (guy), and continues to reflect before time response function of neurons. So you are to micro cell level change and macro behavior--guy up, increased contact reliability and persuasive.

In this paper the temporal area-mainly in the treatment of acute cerebral hemorrhage achieved remarkable curative effect. And in acupuncture treatment of acute cerebral hemorrhage point is to improve the mechanism of the brain nerve cells, corrected the excitability of inhibitory generalization, make be hematoma oppression or hemorrhage of nerve cells stimulate reversible raised or suppressed the nerve cells in the awakening. This hypothesis got clinical electrophysiological confirmed [10 or 11].

Research shows that, salt water group of each group compared with normal value even if there is no difference, but each note brine compared with before has the undulation, its characteristic is note brine slightly larger fluctuation, local adjacent parts fluctuation is lighter after 30 minutes, the basic returned to normal. Specifies that the note saline on its surrounding the influence of neurons simply a repressive function, and with the pure sex oppression of the role, the electrical activity of the neurons lifted soon return to, and it also helps explain the bleeding around the influence of different position is not a simple mechanical pressure caused by role. Note the blood on its surrounding the hematoma after different parts response to pain neurons discharge significantly inhibit one of its local hematoma, inhibition, on the adjacent parts of the heaviest inhibition relatively light. Consider the reasons for local mechanicality oppression is given priority to, and in a certain time persist, adjacent parts in addition to mechanical oppression, and the more hematoma and blood biochemical substances stimulation and local bleeding caused by the secondary vasospasm relevant. Acupuncture can make the role and reduce hematoma by the response to pain neurons excitability of the improved obviously, and immediate effects that acupuncture can improve significantly after the hematoma blood note mechanical oppression and its release chemicals on the surrounding nerve cells, it can reduce the damage blood vessels spasms of ischemia, make the function of nerve cells, both local function recovery activities, also have adjacent parts, and with the role immediate effects for outstanding characteristics.

Two-way adjustment function is an adjustment of balance Yin and Yang, qi role. Rats with existing widely in pain modulation process in two major types of neurons, namely PEN and PIN, both is in pain in the process of production and the acupuncture analgesia, coordinate activities closely cooperate with the two kinds of response to pain neurons. Because of the pain in between exists in the course of a party excitement and other one party suppress opposition of the relationship, so can also will PEN and PIN Yin and Yang between relations as restrict relationship. Head of rat hole acupuncture under different conditions of damaging stimuli have two-way adjustment function, the specific performance in the guy on the incubation period of reflection, physiological conditions make it extended to analgesia, pathological conditions and make its shorten to restore sexual excitement. Can see, acupuncture and function of different functional status of the rats closely related, the rats guy reflection on the state of play different are the best effect. Acupuncture point to head before and after intracerebral hemorrhage rat neurons of electrical activity in response to pain also to have the benign adjustment function, two-way when the pain stimulus makes PEN excited and PIN inhibition, acupuncture can restrain the electrical activity of the PEN and strengthen the electrical activity of the PIN, namely the acupuncture broke both between the original relations, through in the new level to meet the new balance of Yin and Yang state to produce analgesic effect. When bleeding stimulus makes PEN and PIN of the electrical activity is restrained, at the same time between not only balance is broken, and functional state severely inhibit, at this moment the role of acupuncture is restrained, remove resume function, make both even in disease states can still keep a low level balance and the function of the activities in the maximum recovery. This shows the acupuncture to PEN and PIN the electrical activity of the two-way benign adjustment has effect, make the body of the different function in coordination with state, through the PEN and PIN constantly balance activities, make the body toward from harm and tendency of the development direction of normal. From the perspective of modern medicine, acupuncture, and its effect on the head of the stimulated namely changes through the scalp transmission to brain, in the role of the brain cortex cells, which produce oppression and stimulate the bleeding can direct role in nerve cells, so, PEN and the two classes of PIN response to pain neurons and accept that the stimulus from the body, acupuncture function, the influence of hematoma, and then the feeling of information in the wild integrate various information, produce the corresponding excited or inhibit the reaction. This also with our proposed brain may acupuncture in acupuncture point a visceral between-associated feedback regulation center corresponds with the theory. The above results in a certain extent, shows that acupuncture can reduce the damage of neurons bleeding, can promote function of neurons, and points out that the head early resumption point acupuncture for acute cerebral hemorrhage of the early clinical treatment of scientific and necessity.

reference

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