For traditional Chinese medicine "gene id card"

Time: 2017-03-16

Source: China's traditional Chinese medicine

Author: Zhang Mengxue

"Zhao mouth, concave belly button, gastrodia elata outer ring dried ginger skin." Ancient rhyme formulas contain the old it may identify medicinal materials experience accumulation and immortal wisdom. In traditional Chinese medicine system, the identification of Chinese medicinal materials have been on morphological characteristics may mainly based on the drug. However, as more and more into people life of traditional Chinese medicine, Chinese herbal medicine and Chinese medicine products demand is more and more big, the traditional Chinese medicinal materials quality is uneven, even realistic-looking phenomenon has become a problem that nots allow to ignore.

With the promulgation of the 2016 annual national science and technology progress prize, herbal DNA barcode species identification technology into the public eye. By the Chinese academy of sciences, chief researcher of traditional Chinese medicine, the world health organization (who) collaborating centre for traditional medicine director Chen sl led by the scientific research project, the use of molecular biology techniques for traditional Chinese medicine has established the general genetic barcode identification standard, which is of great significance to solve the present Chinese traditional medicine identification problem.

"This is set 'gene id card for Chinese herbal medicine, authenticity can be identified by DNA barcode detection." Chen sl a vivid metaphor.

Traditional Chinese medicine can do "paternity"

Genera close a lot of Chinese medicinal materials, shape, gas and taste are similar, appears to be "real brothers", but in fact their pharmacological efficacy is widely divergent, effective identification of Chinese herbal medicine "pseudo parent-child", to ensure the clinical curative effect of traditional Chinese medicine is significant, DNA barcode species identification technology of Chinese herbal medicine is "paternity" technology of Chinese herbal medicine industry.

"Every kind of traditional Chinese medicine has its own specific" DNA barcode "." China institute of traditional Chinese medicine academy of sciences student xi-wen li is deputy director of the centre for the study of "Chinese herbal medicine DNA barcode species identification system" is one of the main members of the team, according to his introduction, the research team is sign significance to Chinese herbal medicine and its common mixed falsify the DNA sequence of experiment research, according to each trademark DNA sequence characteristics of traditional Chinese medicine output corresponding "DNA barcode", "DNA barcode" can be made into the form of qr code, swept through code, you can identify the authenticity of traditional Chinese medicine.

Research team member, Chinese academy of medical sciences researcher at the institute of medicinal plant Yao Hui said, now, DNA barcode species identification technique has achieved the Internet and mobile client and convenient services, through the multimedia information platform, can realize the traditional Chinese medicine resources of information retrieval, query and matching identification, step, is very convenient and practical.

"2010 edition and 2015 edition of pharmacopoeia of the People's Republic of China" have DNA barcode species identification methods of Chinese herbal medicine as one of Chinese traditional medicine appraisal guidelines." Chen sl mentioned, in the era of the rapid development of Chinese medicine, the traditional identification technology has been unable to meet the demand of market, use of modern means of science and technology to build a new type of traditional Chinese medicine appraisal model is meet the requirements of The Times. "

Identification of traditional Chinese medicine "Renaissance"

Species of Chinese herbal medicine DNA barcode identification system is a technology revolution in the history of traditional Chinese medicine identification, traditional Chinese medicine identification from dependence on experience and subjective feeling of eye view taste, upgraded to rely on the appraisal method of objective gene sequences. The international famous journals the frontiers of biotechnology, referred to as "herbal medicine appraisal from the DNA of the form to the Renaissance".

Xi-wen li said: "we, after 12 years of hard exploration, has completed more than 11000 kinds of 48000 specimens of gene sequencing, established the world's largest database of DNA barcode identification of Chinese medicinal materials, including more than 100 ten thousand strands of DNA sequences, can to China, the United States, Japan, the European Union, South Korea and India, and many other countries natural herbs for species identification. Due to the breakthrough in DNA barcode identification technology, group 2 researchers won" the 3rd international conference on bar code "fully funded, to Mexico to do project report."

As a major research project, Chen sl believes that the main contribution of this project is to establish the "general" standard of identification of Chinese medicinal materials. He explained further, there is no universal identification markers has always been a problem in Chinese traditional medicine JianDingJie, and scientific research team to find the optimal "general" iconic gene as a bar code sequence, namely nuclear gene ITS2 sequences, and published sequences of DNA barcode standards for "Chinese pharmacopoeia" such as book, mark identification science of TCM into the general and standardized era of gene identification.

"From the gene level understanding and the identification of traditional Chinese medicine (TCM), a new way for traditional Chinese medicine to world." Xi-wen li said the Chinese herbal medicine for more complex mixture, this makes it hard for the modern natural science theory understanding and the understanding of traditional Chinese medicine, it also become one of the biggest obstacles to the traditional Chinese medicine to the world, from the Angle of the gene to interpret Chinese medicine conforms to the perspective of natural science, for the Chinese medicine cognition, understanding, acceptance by the world, is of positive significance.

"DNA species identification technology has been incorporated in the British pharmacopoeia." One of the main members of the group, the Chinese academy of medical sciences researcher at the institute of medicinal plant jian-ping han is introduced, the British pharmacopoeia commission sent technicians to come to China to study Chinese herbal medicine DNA barcode identification technique.

Supervision to ensure the quality of traditional Chinese medicine market

At present, Chinese herbal medicine DNA barcode species identification system has been widely applied to the appraisal of scientific research and production, for the supervision and guarantee good quality of Chinese medicinal herbs market has a positive role. , for example, the project's main guangzhou wang ji pharmaceutical co., LTD., one of the DNA barcode species identification technology introduced into the production of herbal tea, the cool tea raw materials used in traditional Chinese medicine and its common mixed falsify rapid identification, accurate identification of DNA barcode, but for the quality control from the source of raw material selection, ensure the raw materials for authentic, stable and consistent.

Chen sl said, as the DNA barcode species identification technology of Chinese herbal medicine is widely recognized and accepted, more workers and traditional Chinese medicine of traditional Chinese medicine industry practitioners put forward higher request to us, we further research direction is on the basis of current research achievement, the application of this technology to the traditional Chinese medicine compound on the identification of species of traditional Chinese medicine compound DNA barcode identification system; We adopt new modern biological technology for species identification of traditional Chinese medicine compound, has made certain achievements, such as the combination of traditional Chinese medicines containing angelica identification database has been basically completed; Hope through the continuous improvement of the Chinese herbal medicine DNA barcode species identification information, in the traditional Chinese medicinal materials quality regulation and traditional Chinese medicine play a bigger role in international communication.